Step 1: Prepare the culture medium: the components are sawdust and wheat bran, and the mass ratio is about 3: 1. The broad-leaved wood chips are usually used. The harder the better, the stamina is sufficient. Sawdust such as poplars and willows is relatively loose, not easy to compact, and has poor stamina. Sawdust of pine, cedar, camphor and cypress, and mildew hardwood sawdust should not be used. If there is no wood chips, you can also use sugarcane dregs, cottonseed hulls, or corn stalk dregs, etc. Weigh the wood chips and wheat bran in proportion, mix and stir well. There are many better formulas for local conditions, and the following introductions are for your reference. use.
â‘ Sawdust medium. 73 kg of wood chips, 25 kg of bran, 1 kg of sugar, 1 kg of gypsum, and 160 kg of water. â‘¡ Bagasse culture medium. Sugarcane bagasse 73 kg, bran 25 kg, stone white paste 1 kg, water 160 kg. â‘¢ Sawdust bagasse culture medium. Sawdust 40 kg, bagasse 30 kg, bran 35 kg, sugar 1 kg, gypsum 1 kg, water 160 kg.
The second step is to make seeds: put the prepared culture material into a jar or mushroom bottle, and pour the bottle a few times while bottling, so as to avoid gaps in the lower part of the bottle, resulting in uneven top and bottom, the appropriate amount is the distance from the bottle The mouth is 3 ~ 5 cm, flatten the upper surface, and then use a bamboo rod with a diameter of about 1 cm, pierce a hole from top to bottom in the center of the bottle, and rotate to exit the bamboo rod to prevent the upper material from being broken. Spread thoroughly with mycelium, stopper cotton stopper plus moisture-proof paper, so that it can also be sterilized with two layers of kraft paper, requiring 1.2 kg pressure for one hour, seed vessel preparation: the wheat bran and water according to the mass ratio of 1: 2 Mix it, mix it evenly, put it in a culture vessel and flatten it slightly. About 10 grams of dry wheat bran can be filled with a 9 cm diameter culture dish. When bottling, the culture medium should not be too thick, or reduce the amount of mixing water by 1: 1.5 is enough.
Step 3: Inoculation: Under sterile conditions, use the inoculated needle to pick up the cultured mycelium with large soybean grains and place it in the wheat bran. Press and put it in an environment of about 26 ℃ for a week, and the white hyphae will basically grow up.
Step 4 Cultivation: The cultivation of Ganoderma can be divided into two stages, preferably two cultivation rooms. The first culture room only requires a temperature suitable for the growth of mycelium 25 ℃ ~ 28 ℃, the bottle should be placed upright, can not be placed horizontally, so as not to fall on the bottle mouth, the culture medium can not be connected to affect the germ, about 10 days In addition to the mycelium extending five or six centimeters into the bottle, white pimple-shaped protrusions are cultivated in the bottle and the surface is formed, which is the seed entity primordium. At this time, the cover should be removed and sent to the second culture room. At this time, the bottle can be placed horizontally. In addition to the appropriate temperature, the relative humidity of the air must not be lower than 70%, preferably 80% ~ 90% In addition, it also requires a certain amount of scattered light and proper ventilation to ensure sufficient oxygen supply, so that Ganoderma lucidum can be differentiated from Ganoderma lucidum normally. Ventilation should be carried out slowly, when the temperature is low, it should be at noon, to avoid the sudden change of temperature caused by Ganoderma lucidum deformity.
After this, after basically cultivating for about 45 to 60 days, the Ganoderma can be harvested.
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