Agilent LC maintenance routine maintenance and care
1, manual injector:
In general, you should pay attention to the use of double distilled water and methanol in the loading state and injection analysis state cleaning; if there is liquid leakage, the cause is likely to be worn or contaminated by the rotor gasket, generally apply for repair or replacement of accessories.
2, drain valve:
When the gasket cannot be completely sealed or leaked here, the gasket is generally contaminated or worn. After removing it, remove the gasket and ultrasonically clean or replace the gasket with isopropyl alcohol.
3. Check valve:
If the pump pressure is unstable or the mobile phase cannot be extracted, there may be a problem with the check valve. Remove the ultrasonic cleaning with isopropyl alcohol. When cleaning, place it in a small beaker according to the direction of its installation. Remember not to install direction. Inverted over wash!
4, workstation:
In the event of a crash, the computer can be restarted; in case of abnormal operation, the computer can be replaced first to test its hardware failure; or the interface can be re-plugged and reinstalled on the machine.
5. Filter: (Agilent LC)
When the chromatographic peak is abnormal, it may be contaminated. Remove the isopropyl alcohol or replace the filter gasket.
6, suction filter:
In special cases, the filter head can be removed to determine whether it is blocked. The mobile phase can also be pumped through the suction filter to determine if it is clogged. If there is any blockage, it can be ultrasonically cleaned with isopropyl alcohol; if the cleaning is not successful, it needs to be replaced.
7, the mobile phase of the pumping requirements:
Liquid phase chromatography alcohol, double distilled water, buffer salts must be filtered;
Degassing of the mobile phase is essential (using suction filtration, ultrasonic degassing, etc.)
8, the circulation pool:
This component may be contaminated when the peak is not normal. After detachable, remove the gasket and wash it with isopropyl alcohol (can be operated according to the instructions or apply for repair).
9, pump head:
If the pump head leaks or other faults occur, it is generally necessary to apply for repair (such as leakage, or replacement of the plunger rod and its gasket).
Pump pressure is unstable:
1. There are bubbles in the pump head - mobile phase degassing / large flow flushing pump / pumping mobile phase
2, check valve failure - cleaning If the above operation can not be resolved, you can use isopropyl alcohol to flush the flow (no column wash, directly into the detector by the manual injector), flow rate of 3-4ml / min, rinse for 50 minutes Left and right, then re-install the column, replace the mobile phase balance, so that can not solve the pump pressure fluctuations, you can apply for replacement parts.
10. Leakage treatment: (Agilent LC)
The C18 column cannot enter protein samples, blood samples, or biological samples.
Pay attention to the pH range of the column, and do not inject samples of strong acid and alkali, especially alkaline samples.
If the instrument is not used for a long time, the column should be taken up and sealed with a plug. Note that the column should not be stored with pure water, but should be stored with an organic phase (such as methanol) because pure water is prone to mildew.
Flow path blockage problem: The pressure caused by the blockage is too large, and it is checked by the pre-column → filter in the mixer → line filter → check valve, and cleaned. Cleaning method: 1 Wash with human propanol as solvent 2, place in isopropyl alcohol, ultrasonic cleaning, 3 clean with 10% dilute nitric acid
11. Use of buffer salts:
When the mobile phase contains salt, it must be flushed after the experiment is completed;
First rinse with water, open the drain valve and use the PURG button to convert the original salt-containing mobile phase, then close the drain valve and open the pump to flush all the flow paths for more than 45 minutes. Then replace the mobile phase with water and methanol mixture to flush all the flow paths. 30 minutes (this step can generally be omitted, according to the experiment), after the Zui, replace the pure methanol to rinse all the flow for more than 45 minutes.
12. Pump head flushing;
Rinse 3-5 ml with distilled water and pure methanol, respectively, using a needle and a syringe in the spare part.
If the mobile phase does not contain salt, the machine can be periodically rinsed and maintained, depending on the number of experiments.
Manual injector rinse;
Also use the needle tube in the spare part and the special flushing needle to flush the loading state and the injection state of the manual injector separately with 3-4 ml of distilled water, and then rinse 3-4 ml of pure methanol. Make sure that all the flow paths are filled with pure methanol every time you complete the experiment!
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