Whole transcriptome sequencing provides a target molecule for studying the role of lncRNAs in radiation-induced intestinal injury
Grateful to have you, all the way! At the beginning of 2019, Yunxu Bio with all the staff expressed their sincere greetings to the new and old customers who have been caring and supporting the company's development. One yuan starts again, Vientiane is updated! In a twinkling of an eye, we ushered in 2019, standing in the new historical starting point of the new era, looking back on the past 2018, constantly innovating and harvesting fruitful results; Looking forward to 2019, we have a long way to go but confidence has multiplied!
Good news! Recently, Yun Qiang's research by Liu Qiang of the Institute of Radiation Medicine of the Chinese Academy of Medical Sciences revealed changes in the expression of lncRNAs after jejunal irradiation in mice, providing a target molecule for studying the role of lncRNAs in radiation-induced intestinal damage. The results of the study were published in "J Cell Mol Med" ( IF: 4.302 ) under the title " Analysis of changes to lncRNAs and their target mRNAs in murine jejunum after radiation treatment ". If you happen to read this article and pay attention to our previous public account, you will have a feeling of deja vu, yes! Not long ago, the research team published an article on the expression of circular RNAs (Analog of Circular RNAs Altered in Mouse Jejuna After Radiation , IF: 5.5 ), also from the Liu Qian research team, the lncRNA and circRNA data in two articles. All are from the whole transcriptome sequencing service provided by Cloud Sequence. It truly reflects the three sets of data (circRNA lncRNA and mRNA), a multi-product, super cost-effective, and short-post high-scoring articles.
So let's take a look at the ingenuity of whole transcriptome sequencing through the contents of two articles !
Article 1: Study of lncRNA expression profile after jejunal irradiation in mice (IF 4.302)
research content:
1. The expression profiles of lncRNA and mRNA in the radiation group and the control group. The clustering diagram showed the up-and-down changes between groups and groups. The Venn diagram showed the overlap of lncRNA and mRNA in the irradiation group and the control group. The volcano map showed the difference. Express the situation.
2. The relative amount of lncRNA and mRNA in irradiated mice compared to the control group. Histograms show up- and down-regulation of lncRNA and mRNA in irradiated mice, and Venn diagrams show target gene predictions for lncRNA to define the function of lncRNA.
3. qPCR verification of differential lncRNA molecules. For an article on spectrum, the results of differential lncRNA verification only need to be qPCR.
4. Differentially expressed lncRNA molecular function prediction, GO and KEGG pathway analysis showed that the predicted genes are mainly involved in the VEGF signaling pathway.
5.lncRNA-miRNA-mRNA regulatory network analysis, find the lncRNA that plays a role in the sponge mechanism, and the target genes it regulates.
Article 2: Study of circRNA expression profiles after jejunal irradiation in mice (IF 5.5)
1. What is the expression of circular RNA? What are the differences in expression?
High-quality sequencing services, professional biometric analysis, cloud-order biology make ring research easy, this is an advertisement. High-quality sequencing technology accurately detects the expression of each loop. The biometric analysis visualization gives all the circular RNA in the jejunum of mice after radiotherapy from a macroscopic perspective (chromosomal distribution, loop position). The expression of molecules.
On this basis, which circular RNA molecules are differentially expressed? Here, the volcano map and the Venn map are all available.
2. qPCR verification of differential circular RNA molecules, as in the previous article, qPCR verification is essential.
3. Differentially expressed circular RNA molecular function prediction, GO analysis and Pathway analysis as an essential part of functional prediction are also covered in this study, including GO, entry, catalysis, cytokine and cell secretion are rich set. KEGG analysis predicted that the target gene of differential circular RNA was associated with MAPK signaling pathway (Cacna1e, Mras, Prkcb, Ptpn7), and recent reports indicate that EGF-mediated activation of Ras/Raf/Mek/Erk/MAPK signaling pathway Intestinal stem cell proliferation is associated.
4. Sponge mechanism predicts ceRNA network map. According to the transregulation of the sponge mechanism, the network includes 42 up-regulated and 48 down-regulated circular RNA molecules, as well as predicted 22 target gene mRNAs that bind to each other.
to sum up
By comparing the two articles, you can find that in the new field, with the high-throughput sequencing of new molecules, it is a means to efficiently and quickly publish articles. The new molecules lncRNA and circular RNA, both in the transcriptome field, remain the darling of research articles and funds for two years. The team of Liu Qiang's team realized two sequencing of one article through the whole transcriptome sequencing of Yunxu Bio . The cumulative impact factor was about 10 points, achieving a super high cost performance! Seeing such an affordable and incomparably efficient article-writing strategy, are you also excited? Then don't hurry and contact us.
The 2019 National Natural Application is just around the corner. Xiao Bian believes that the three hot spots we summarized last year (RNA methylation, exosomes, and circular RNA) will still be a hot spot in 2019. Interested can refer to:
The hotspot of natural research in 2018: a deep analysis of circular RNA research
2018 National Nature Research Hotspot 2: In-depth analysis of RNA methylation research
Hotspots of Natural Research in 2018: Deep Analysis of Exosomes
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Whole transcriptome sequencing
Circular RNA sequencing
LncRNA sequencing
m6A RNA whole transcriptome methylation sequencing
m5C RNA whole transcriptome methylation sequencing
m1A RNA whole transcriptome methylation sequencing
RNA pull down
RIP sequencing
Past review:
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