Important application of MED64 system: visual signaling pathway recombination in the rat model of Cacna1f gene mutation

August 31, 2022

Instrument Application Manual Volume 6
Important application of MED64 system: visual signaling pathway recombination in the rat model of Cacna1f gene mutation
Ye Tao, Tao Chen, Ji-jing Pang, ZuoMing Zhang, etc.
unit:
Department of Clinical Aviation Medicine, Fourth Military Medical University, Xi'an, China;
Tangdu Hospital, Fourth Military Medical University, Xi'an, China;
University of Florida College of Medicine, Gainesville, Florida
Keywords : multielectrode array (multi-array system, MEA) system; MED64; retina (retina); congenital resting night blindness (CSNB); Cacna1f gene; calcium ion channel
The research team from the Fourth Military Medical University and the University of Florida Medical School studied the electrical signal transmission characteristics in the retina of the Cacna1f gene- mutated rat model through advanced electrophysiological techniques ( multi-array electrode system ), and described the congenital static The underlying pathological mechanism of night blindness (CSNB) .
The Cacna1f gene encodes the a1 subunit of the Cav1.4 calcium channel. The variation of the 1f gene can induce the abnormality of calcium ion flux by affecting the function of calcium channel , causing abnormal secretion of neurotransmitters in nerve cells, which greatly affects breastfeeding. The generation and transmission of animal visual signals. These mutations in the Cacna1f gene are closely related to iCSNB disease (incomplete x-linked congenital resting night blindness ). It is a hereditary severe night vision loss that will seriously affect the quality of life of patients. The research on iCSNB has been very extensive. Through the rat model of mutation, the researchers have carried out a lot of research on iCSNB disease, but due to the limitations of research techniques, the variation of Cacna1f gene is on the visual network.
Little is known about the effects of action potential signals in membrane ganglion cells. The researchers used the MED64 multi-array electrode system (MEA system) from Alpha MED Japan to conduct retinal research. The MED64 system is capable of conducting research on the retina. The MED64 system continuously collects the neuromotor action potentials of retinal cells through a densely distributed 64 microelectrodes, and provides a map of the distribution of neurophysiological functions of the neurons, thus providing valuable clues for the interconnection of retinal neurons. Thanks to the holistic research features and non-invasive advantages of the MED64 system, this technology solves the gap between in vitro signal recording and in vivo recording studies, especially in ion channel-related electrophysiological studies. potential.
result:
Typical electrical signals were successfully obtained from retinal samples of wild-type and Cacna 1f mutant rats (CSNB mice). The a-wave amplitude induced by light-induced in the retinal samples of CSNB rats gradually decreased while b-wave disappeared. The application of 100 uM of 4-amino-4-phosphobutyric acid did not retard the production of a-wave. The increase in spontaneous electrical signals and the decrease in the frequency of light-induced electrical signals reflect a decrease in the ability and stability of retinal ganglion cells to capture light signals. In addition, the 'ON PATHWAY' is always inexplicably associated with retinal ganglion cells, and CSNB rats may prefer to select the 'OFF pathway' for signal transmission. In the electrical induction experiment, the retinal samples were induced by electrical pulse, and the long delayed reaction intensity of RGCs was weaker in CSNB rat samples than in wild type rat samples.
in conclusion:
Using the MED64 system (multi-electrode array system (MEA), multi-electrode array system), the researchers found evidence that the retinal function of the Cacna1f mutant mouse (CSNB rat) was altered in the optical signaling pathway. The researchers found that dysfunction of photoreceptor neurotransmitters and loss of photoreceptor function occur in CSNB rats, which may be reversible.
Two different sizes of MED64 electrodes. A. Electrodes for recording field potential signals over a wide range; B. Electrodes for recording the association between spontaneous spikes and small area nerve cells.
A is a light-induced a-waves map of wild-type and CSNB-type rats;
Difference in amplitude of light-induced a-waves in B Wt and CSNB rats;
The b-wave present in C Wt can completely remove 100BM APB, and can be recovered after washoff;
Original reference: Visual Signal Pathway Reorganization in the Cacna1f Mutant Rat Model
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