Nuclear Reference PCNA Antibody - PCNA Monoclonal Antibody for Nuclear Nucleus Reference

Proliferating Cell Nuclear Antigen (PCNA) was first discovered and named by Miyachi in the serum of patients with SLE (systemic lupus erythematosus) in 1978, and was named for its presence only in normal proliferating cells and tumor cells. PCNA is a protein with a molecular weight of 36KD, which is synthesized in the nucleus and is present in the nucleus and is an auxiliary protein of DNA polymerase. Because of its stable expression in the nucleus, its antibodies are widely used in internal reference antibodies of nuclear proteins.

How to choose the appropriate nuclear internal reference antibody?

The commonly used total cellular protein is GAPDH and the cytoskeletal protein β-Actin or β-Tubulin. For some plant samples, a special plant Actin protein is required as an internal reference. When the experimental sample is only a nuclear protein, not a total cellular extract, a histone H (Histone H), or a proliferating cell nuclear antigen (PCNA) may be used as a nuclear reference antibody. In addition to these, other common nuclear protein internal references are K70, K80, Lamin A and B. In some literature reports, Erk2, TATA binding protein (TBP), c-Jun, c-Fos, etc. are used. However, the problem to be noted is that the choice of nuclear protein internal reference needs to consider the actual experimental environment. For example, in the experiments involving cell proliferation, c-Jun is not suitable for internal reference because of its own expression changes; in the apoptosis experiment, TBP, Lamin Etc. is also not suitable as an internal reference.

In addition, we also need to select the appropriate nuclear internal reference according to the size of the protein of interest. We recommend that the internal reference should have a molecular weight difference of more than 5KD, so you need to select the appropriate internal reference according to the molecular weight of the protein. The detection molecular weight of PCNA is about 28kD, while the detection band of Histone H3 is about 18kD. Therefore, PCNA is more suitable for detecting target proteins in larger WB experiments, while Histone H3 is not suitable for 13kD-23kD size target proteins. Internal reference . In addition, although PCNA is one of the most suitable nuclear internal reference antibodies, under certain conditions, some factors affecting proliferation will lead to changes in PCNA content between samples, and it is not suitable as an internal reference.

The initial purchase of Abbkine's PCNA antibody was used to make WB, and the titer was quite high. Later, we used tissue blocks as samples for co-localization. I tried to use this antibody to locate PCNA and do immunohistochemistry. WB and IHC are very good. ——One client of the Central South University College of Sciences

In addition, we also need to consider our own needs, especially the needs of experimental applications. In general, the more types of applications, the greater the choice in use, and the monoclonal antibodies from mouse sources generally have better specificity, stability and potency than polyclonal antibodies. In addition, the titer of the antibody is also an important aspect to examine the cost performance of the antibody, which is equivalent to the dilution ratio in practical use. A high titer of 100 μl of PCNA antibody (if the dilution ratio of WB assay is 1:5000, the final use solution is equivalent to 500 ml), 1 ml of β-Tubulin antibody with lower potency (if the dilution ratio of WB assay is 1) : 200, the final use liquid is equivalent to 2ml) more cost-effective. Therefore, when selecting antibodies, not only the amount of antibody needs to be considered, but also the titer of the antibody, that is, the recommended dilution ratio for different applications.

Why choose to recommend Abbkine brand PCNA antibodies?

There are many brands that supply PCNA antibodies, such as Abcam, Santa Cruz, Cell Signaling, Sigma, and even Millipore, R&D, Novus, BD, etc. Here is a simple comparison of several imported brands:

Brand	Item number	2013 offer	specification	App types
	Ab29	¥4418	100μg	WB, IP, IHC
	2586S	¥2997	100μl	WB, IF, IHC, FC
	SAB2502099	¥3299	100μg	WB, ELISA
	A01040	¥1800	200μl	WB, IHC

In addition, some domestic suppliers can also provide PCNA antibodies, but there are insufficient brands and quality assurance. The domestically packaged PCNA antibody is insufficient in quality stability, and its valence is also lost after being packaged. Some PCNA antibodies originating in domestic laboratories have better detection activity, but there are gaps in application types, and it is difficult to control the stability between batches. However, only a small amount of native antibodies in China are also Polyclonal antibody. If you want to buy a PCNA nuclear reference antibody that is more cost-effective and also an imported brand, then Abbkine may be one of your important considerations. We recommend Abbkine's PCNA nuclear internal reference antibody Anti-PCNA Monoclonal Antibody (1D7) (Cat. No. A01040).

The antibody monoclonal antibody has the clone number 1D7. Its antigen is a specific and conserved sequence of human PCNA protein, which can be used for WB internal reference of mouse, human and rat samples. In addition, the titer of this antibody is very high, the recommended dilution ratio for WB experiments is 1:1000-1:10,000, and even customers have reported higher dilution ratios.

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The PCNA internal reference antibody immunohistochemistry also has a very good effect, and is an antibody product which is very suitable for immunohistochemistry to stain and localize the cytoskeleton. In addition, some customers reported that the antibody was used for fluorescent staining of tissues, and unexpected effects were also obtained.

Abbkine also offers large-package 1ml and larger custom-made PCNA-tagged antibodies, making it ideal for immunological experiments where internal reference validation or calibration is often performed. For the convenience of customers, Abbkine is available in stock in China. Abbkine provides global product quality tracking and after-sales service and protection for the Chinese market. If you have any problems during the use, please contact your local agent Ai Meijie Technology Co., Ltd. directly.

The American Abbkine brand is a brand that provides cost-effective immunology and cytology research products and solutions. Amy Jie introduced it to China as its regional agent in China, and will provide customers with the most cost-effective routine immunological reagents, such as A tag antibody, an internal reference antibody, a conventional primary antibody, and a full range of secondary antibodies, antibody detection substrates, and related kits. At the same time, as a Chinese agent or regional agent of many international famous brands, Aimei can provide you with a series of laboratory solutions. If you are interested in the above products, please call the free customer line 400-6800-868 to 801/802/803 or 027-84454412/4876 to contact Aimei Technology Co., Ltd. for product information and complete experimental solutions.

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