Transportation conditions : normal temperature transportation
Item: 51029: 50 reactions
51030: 100 reactions
Storage conditions: at room temperature (15-25 ℃), -20 ℃ storage digestive please
|
Component | 50 times | 100 times |
Adsorption column and collection tube | 50 each | 100 each |
Lysate | 12 mL | 24 mL |
Sediment | 4.5 mL | 9 mL |
detergent | 9 mL | 18 mL |
Eluent | 3mL | 6 mL |
Digestive juice | 1.2 mL | 2.4 mL |
Instruction manual | 1 copy | 1 copy |
product description
BIOG DNA Swab Kit is a kit developed by Changzhou Baidai Biotechnology Co., Ltd. for the extraction of DNA from buccal swabs, genitourinary swabs, sputum, etc. The BIOG DNA Swab Kit uses the latest high-quality imported ion membranes, and the lysate and eluent are optimized several times to efficiently separate DNA. Compared with other brands, the extracted DNA has higher yield, higher purity, and maximizes the removal of impurities such as proteins, pigments, lipids, etc., and can be directly applied to PCR, real-time PCR and various enzyme digestion tests. .
Steps
a) Precipitate: 4.5 mL was added to 25.5 mL of absolute ethanol; 9 mL was added to 51 mL of absolute ethanol.
b) Washing solution: 9 mL of 21 mL of absolute ethanol was added; 18 mL of 42 mL of absolute ethanol was added.
c) If the prepared precipitate and washing solution are precipitated, they can be dissolved at 37 ° C, shaken and used.
a) Swab: Add 800 μL of normal saline to a 1.5 mL centrifuge tube, place the sample collected by the swab in physiological saline, rinse for 20 seconds, completely detach the cells, and squeeze the liquid on the swab by the wall of the centrifuge tube. Centrifuge at 12,000 rpm for 5 min, discard the 700 μL supernatant, and leave 100 μL of the supernatant remaining, and mix well for 15 seconds.
b) sputum: Add 4 times volume of 1M NaOH to the collected sputum, leave it at room temperature for 30 minutes, shake it once every 10 minutes, centrifuge at 12,000 rpm for 5 min, discard the supernatant, add 0.5 mL of normal saline, and mix. Centrifuge at 12,000 rpm for 5 min, discard 400 μL of supernatant, leave 100 μL of supernatant, and mix well for 15 seconds.
9. Remove the adsorption column, place it in a new 1.5 mL centrifuge tube, add 30-50 μL of eluate, let stand for 3 min, centrifuge at 12,000 rpm for 4 min at 2 °C, and collect the DNA solution. The extracted DNA can be used in the next experiment or at -20 °C.
Precautions:
The kit can be transported at room temperature, stored at room temperature (15-25 °C), valid for 12 months, and stored in digester at -20 °C to avoid repeated freezing and thawing.
QC
This product has passed strict quality inspection and proved that there is no biological pollution.
Note : This product is for scientific research only.
Changzhou Baidai Biotechnology Co., Ltd.
Address: New Oriental Logistics Park, No. 51 Dongfang East Road, Changzhou Tel: 0519-88050392 Fax: 0519-83382790
Freeze-Dried Fruit,Freeze Dried Organic Fruit,Organic Freeze Dried Fruit,Freeze-Dried Fruit Powder
Shaanxi HuiKe Botanical Development Co.,Ltd , https://www.oasis-hk.com