| ã€Purpose】  Observe the autonomous behavior, inquiry behavior and tension of experimental animals in new and different environments.  ã€experiment equipment】  The experimental device consists of two parts: the market reaction box and the data automatic acquisition and processing system. Rat's open field reaction box is 400mm high, the bottom side is 1000mm long, the inner wall is black, and the bottom surface is divided into 25 small squares of 4cm×4cm.  A digital camera is placed at the m, and its field of view covers the entire interior of the market. The lighting in the market is full artificial lighting. It can be artificially set to “daytime†and “nightâ€. During the day, four energy-saving lamps on both sides of the wall emit about 2001ux illumination to simulate, and the night is illuminated by the infrared light source of one side wall. Equipment such as experimenters and computers are located in another room to reduce interference with animals, and laboratory background noise is controlled below 65 dB.  The reaction field of the mouse field is 25-30 cm high, the bottom side is 72 cm long, the inner wall is black, and the bottom surface (software) is divided into 64 small squares. The automatic data acquisition and processing system is the same as above.  [Experimental principle]  Open field experiment  Test), also known as open box experiment, is a method to evaluate the autonomous behavior, inquiry behavior and tension of experimental animals in new and different environments. The spontaneous behavior and inquiry behavior of experimental animals in unfamiliar environments were reflected by the frequency and duration of certain behaviors of experimental animals in a novel environment, and the tension was reflected by the number of urine stools.  [Laboratory Animals]  Rat or mouse.  [Experimental methods and steps]  The experiment was carried out in a quiet environment. Place the animal in the center of the bottom of the box while taking pictures and timing. After watching for a certain period of time, stop the camera. The observation time can be based on the experiment, usually 3~5.  Min. Clean the inner and bottom surfaces of the square box to prevent the last information left by the animal (such as the animal's large, urinal, smell) from affecting the next test result. Replace the animal and continue the experiment.  [observation index]  According to the computer software design, different observable parameters are different. For example, the animal stays in the central grid within the unit time, the number of grids crossed by one limb is horizontal, and the number of hind limbs is vertical, retouching, urine. The number of times; speed of movement, distance of movement, time of rest, distance of movement along the side, distance of central movement, etc.  ã€Precautions】  (1) Animals at 24  There is a period of activity in h, so each experiment should be completed within the same time period.  (2) The experiment shall be carried out in a laboratory where the sound insulation, light intensity and temperature and humidity are appropriate and consistent.  (3) Clean the experimental equipment between the two experiments to avoid the last remaining animal information affecting the results of the next experiment.  [Evaluation of the experimental method of the market]  The open field experiment is a common method to evaluate the autonomous behavior, inquiry behavior and tension of experimental animals in new environments. Easy to operate and reliable. In particular, the introduction of computer technology and image processing technology has greatly increased the detection index of animal behavior analysis, and has many advantages such as high efficiency, non-invasiveness, sensitivity and objectivity. |
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