The incubation period is the measurement data, and the variance analysis can be used. However, the homogeneity test of variance is first performed according to the procedure of analysis of variance. The variance is calculated, the F value is calculated , and the comparison method is used to compare the averages of multiple experimental groups and one control group. Perform appropriate variable conversion on non-normal or variance data. After the normal or variance requirements are met, the converted data is used for statistics; if the variable has not yet reached the normal or variance, Use the rank sum test to perform statistics. Counting data, for example, the number of crossing stations, can be tested by X2 . If the total number of four-grid tables is less than 40 , or the total number of cases is equal to or greater than 40, but the theoretical number is equal to or less than 1 , the exact probability method should be used instead.
Morris  The experimental data of the water maze is more complicated, and its statistical analysis is also more difficult. In particular, there are many problems in the statistical analysis of the escape latency measured by the rats in the search for hidden platform tests. In the past studies, some domestic literatures directly calculated the average escape latency of each day and then performed t- test or analysis of variance, or according to the escape latency of rats, the stability was stabilized 3 days after the navigation test , only for the last 3 days. The average escape latency was analyzed by t test or analysis of variance. Some studies conducted a one-way analysis of variance for the escape latency of 1 to 5 days . We believe that the above statistical methods are not correct. A more obvious error is that the average escape latency of the last 3 days of stabilization tends to represent the memory level. The first problem encountered is that the rats in the physiological state only show this, while the experimental memory impairment group tends to be 3 days later. The escape latency has continued to decline. The escape latency of normal young and early-aged rats tended to be stable in the last 3 days , but the escape latency of rats in experimental injury group was higher due to learning and memory impairment, and the escape latency was still decreasing on the 3rd to 5th day. It does not tend to be as stable as the normal control group. Not only that, but also the above statistical methods for the misunderstanding of the statistical characteristics of the escape latency data measured in the hidden platform test. The escape latency in the hidden platform test has the following characteristics: 1 series repeated measurement design, Morris water maze experimental criteria (4) is the data obtained from repeated measurements on the same subject at different time points after giving a certain treatment. ( Escape latency ) ; 2 influences the evasion latency ( independent variable ) statistical analysis results, at least animal group, detection time point and other effect factors, there are interaction effects between effect factors; 3 different time points of the same test animal There is a high degree of autocorrelation in the measured escape latency; 4 there is censored data ( maximum latency ) . Accordingly, the escape latency of the hidden platform test in the Morris water maze should be based on the multivariate analysis of variance of the repeated measures data of variance (ANOVA) combined with the survival analysis method of the censored data, which is commonly used in foreign literature reports. The method further analyzes the mean value of the latency, with the number of days as the independent variable, the incubation period or the swimming speed as the dependent variable.
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