Safety specifications for animal surgical instruments and their cleaning, maintenance and disinfection protocols

Laboratory safety

During the process of cleaning the surgical instruments during and after the operation, the operator directly contacts various instruments, and is easily exposed to sharp objects, and the blood, body fluids, secretions and the like of the animal are easily exposed to the human body, so appropriate protection is adopted. necessary.

(1) Eye protection: Wear protective glasses, eye protection or protective mask properly to prevent blood or washing liquid from splashing, and effectively protect the eyes or facial skin from infection;

(2) Oral and head protection: wear a hat and a mask, and the quality should not be too thin or too small. If it is splashed by blood during work, it should be replaced immediately;

(3) Protection of the trunk and lower limbs: Wear experimental clothes and protective shoes, and it is best to have water-repellent effect. The experimental clothes are enough to cover the front side of the operator's torso and the upper arms. Once damaged or leaking, they must be replaced immediately;

(4) Hand protection: Always wear gloves to reduce the risk of infection of sharp skin piercings. If you are accidentally injured by a sharp device, you must keep calm, quickly remove the hands and gloves as usual, then sideways and squeeze the injured part from the proximal end to the telecentric end, so that part of the blood is discharged to relatively reduce the pollution. To the extent that it is repeatedly squeezed, rinse with running water, and disinfect the injured area with iodine or alcohol.

Rui Ward animal surgical instruments cleaning

(1) After the end of the animal operation, first soak for 5-10 minutes with the multi-enzyme cleaning agent, so that the dirt remaining in the surface of the instrument and the joint of the mechanical joint is decomposed and softened, and should not be directly immersed in hot water, alcohol, disinfectant. Or preservatives, which will cause mucus, blood or other body fluids to solidify, affecting the next cleaning;

(2) Use the flowing distilled water (do not use physiological saline) to flush away the visible blood stains. During the washing process, the joints of the instruments should be opened to facilitate thorough cleaning;

(3) If the procedure is rusted after being used for a long time, use a special rust remover for manual rust removal treatment. Similarly, during the brushing process, the joints of the device should be opened and fully brushed;

(4) After the washing or brushing is completed, the shaft of the surgical instrument is fully opened, placed in the cleaning basket of the automatic cleaning machine or the ultrasonic cleaning machine according to the type, and automatically cleaned, and the multi-enzyme cleaning agent is added into the cleaning liquid (temperature Usually controlled at 30 ~ 40 ° C);

(5) After cleaning, put it in an oven at about 50 °C or dry at room temperature to avoid contact with moisture or liquid for a long time;

(6) After drying, put them in the surgical instrument box or package them in special cotton according to the type of the instrument.

Special Note: For microscopic, fine surgical instruments or scissors, try not to brush with brush, or use excessive force or pressure to operate, which may cause damage to fine instruments or cutting edges. It is recommended to wipe gently with a soft cotton cloth. Clean; when storing, protect the tip of the instrument with a rubber boot and store it separately.

Maintenance of Riward Animal Surgical Instruments

The performance of animal surgical instruments is directly related to the success or failure of the operation or the health of the animal. At the same time, if it is left unmaintained for a long time, it will shorten its service life. Therefore, regular maintenance is also an essential task. It is recommended to follow the steps below. :

(1) Wiping the surface dust;

(2) After soaking for 30 minutes with a rust remover, rinse with distilled water (not to use physiological saline). Fully open the joint portion of the instrument during soaking;

(3) Drying with a soft absorbent towel or cotton cloth or drying the device in an oven at about 50 °C;

(4) After drying, wipe evenly with paraffin oil;

(5) After the above steps are completed, they are placed in the surgical instrument box or packaged in special cotton according to the type of the device.

Special Note: For microscopic, fine surgical instruments or scissors, try not to brush with brush, or use excessive force or pressure to operate, which may cause damage to fine instruments or cutting edges. It is recommended to wipe gently with a soft cotton cloth. Clean; when storing, protect the tip of the instrument with a rubber boot and store it separately.

Disinfection of Rivet animal surgical instruments

In order to ensure the safety of animal experiments, to prevent animals from being infected, and to ensure the personal safety of the experimental operators, surgical instruments must be disinfected and sterilized before each use. It is noted that before disinfection and sterilization, the surgical instruments should be cleaned to completely remove the blood stains, grease and other dirt on the instruments, so as not to affect the disinfection and sterilization effect.

(1) Disinfection: It is usually immersed in 2% glutaraldehyde distilled water solution for 10-30 minutes for disinfection. It can also be used as disinfectant such as Xinjieering solution, 70% alcohol and bus disinfectant. Before soaking and disinfecting, wipe the grease on the device; 2 the equipment to be disinfected must be immersed in the solution; 3 the device with shaft joint (such as scissors), the shaft joint should be fully opened; 4 before further sterilization or use Rinse the solution with sterile saline, then rinse off with sterile distilled water to prevent damage to the animal tissue.

(2) Sterilization:

A. Dry heat sterilization method: This method is the best sterilization method, which has the least damage to the equipment and is suitable for sterilization of metal and glass instruments. The specific method generally involves packaging the device items to be sterilized and then baking them in an electric oven, that is, heating to 160-170 ° C (up to 200 ° C) for 1-2 hours. In particular, do not place the instrument too tightly to avoid obstructing the air circulation. It is recommended to use a metal sterilization box or other high temperature resistant cloth to package the equipment to be sterilized. Never use oil paper, wax paper, cotton and other packaging equipment, it is easy to be roasted. Burning coke, danger occurs;

B. Autoclaving: Another common surgical instrument sterilization method in the laboratory, but it has a certain degree of damage to the device, which will shorten its service life. The specific method is generally: after the equipment items to be sterilized are packaged, they are placed in a high-pressure steam pot. When the steam pressure reaches about 1.05 kg/cm2, the temperature of the water vapor can be raised to 121 ° C for 20-30 minutes; The reminder is to pay attention to the water level in the sterilizing pot, and the water should be added before sterilizing to prevent the drying pot from being sterilized;

C. Boiling disinfection method: This method is less applied, because it has the greatest damage to the surgical instrument, it will significantly shorten its service life, and is suitable for metal instruments, syringes, glass products and rubber-like equipment. The specific method is to put the packaged instrument into the boiling sterilizer, boil at 100 ° C for 15-30 minutes (killing the general vegetative body) or 1-2 hours (kill spores or spores).

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