Rat (G-CSF) ELISA kit technology principle
Samples: Serum, plasma, tissue and related fluid adaptation species: Human, Rat, Mouse, Monkey, Rabbit
Application: Biotechnology detection method: Enzyme linked detection / ELISA
Detection limit: Scientific research experiment supplier: Qiao Yu Biota: 100
Specifications: 96T/48T
Rat (G-CSF) ELISA kit Technical principle Rat granulocyte colony-stimulating factor (G-CSF) ELISA kit Rat granulocyte colony-stimulating factor (G-CSF) ELISA kit technology supports rat granulocyte colony stimulation Factor (G-CSF) ELISA Kit Instructions Shanghai Qiaoyu Biotechnology Co., Ltd. specializes in providing various species, various series of ELISA kit test kits, ELISA kit technical support, ELISA kit technology principle, ELISA analysis Kit instructions. All of the Elisa kits of Qiao Yu provide free testing services, providing complete technical guidance from the whole process of specimen collection, preservation, pre-test, experiment, data analysis, etc., specializing in imported sub-packages and original and domestic Elisa kits. Quality Assurance! Welcome new and old customers to call to order!
Product Name: Rat (G-CSF) ELISA Kit Technical Principles English Name: Rat Granulocyte Colony Stimulating Factor, G-CSF ELISA Kit
Product No:QY-SZ2078
Product traits: liquid product use: scientific research experiment product specifications: 96T/48T
Customer Service Phone QQ Email @qq.com
Steps:
1. Mix all reagents thoroughly before use. Do not allow the liquid to generate a large amount of foam, so as to avoid adding a large amount of air bubbles during the loading, resulting in errors in the loading.
2. The number of slats required is determined by the number of samples to be tested plus the number of standards. Multiple holes are recommended for each standard and blank hole. Each sample is determined according to its own quantity, and it is possible to use the double hole as much as possible to make a double hole.
3. 50 ul of the diluted standard was added to the reaction well, and 50 ul of the sample to be tested was added to the reaction well. Immediately add 50 ul of biotinylated antibody. Cover the membrane, mix gently by shaking, and incubate for 1 hour at 37 °C.
4. Remove the liquid from the well, fill each well with the washing solution, shake for 30 seconds, remove the washing solution, and pat dry with absorbent paper. Repeat this operation 3 times. If washing with a washer, the number of washings is increased once.
5. 80 ul of affinity streptavidin-HRP was added to each well, and the mixture was gently shaken and incubated at 37 ° C for 30 minutes.
6. Remove the liquid from the well, fill each well with the washing solution, shake for 30 seconds, remove the washing solution, and pat dry with absorbent paper. Repeat this operation 3 times. If washing with a washer, the number of washings is increased once.
7. Add 50 ul of substrate A and B to each well, mix gently by shaking, and incubate for 10 minutes at 37 °C. Avoid lighting.
8. Remove the microplate and quickly add 50 ul of stop solution. Immediately after adding the stop solution, the results should be determined.
9. The OD value of each well was measured at a wavelength of 450 nm. Reagents and equipment that are needed but not provided
Standard specification microplate reader
2. High speed centrifuge
3. Electric heating incubator
4. Clean test tube and Eppendof tube
5. Series adjustable pipettes and tips, multi-channel pipettes are preferred when testing more samples at a time
6. Distilled water, volumetric flasks, etc.
Calculate <br> with the concentration of the standard as the abscissa and the OD as the ordinate. Draw a standard curve on the coordinate paper, and find the corresponding concentration from the standard curve according to the OD value of the sample; multiply by the dilution factor; or Calculate the linear regression equation of the standard curve by the concentration of the standard and the OD value. Substituting the OD value of the sample into the equation, calculating the sample concentration, and multiplying by the dilution factor, is the actual concentration of the sample.
Kit performance
1. Sensitivity: The minimum detection concentration is less than the No. 1 standard. The linearity of the dilution. The linear regression coefficient of the sample and the expected concentration correlation coefficient R value was 0.990.
2. Specificity: Does not react with other human cytokines.
3. Repeatability: The coefficient of variation between the plate and the plate is less than 10%.
Result judgment and analysis
1. Instrument value: read the OD value of each hole on a microplate reader with a wavelength of 450 nm.
2. The absorbance OD value is the ordinate (Y), and the corresponding HSP-70 standard concentration is the abscissa (X). The corresponding curve is obtained. The HSP-70 content of the sample can be converted from the standard curve according to the OD value. The corresponding concentration.
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If it is not possible to judge by experimental data only, you can send the kit back to us for post-sale testing.
If it is the quality of the kit itself, you can return it for you;
If it is a problem with the experimental operation, the technician can give you some suggestions for improvement.
Tips: Not for clinical treatment.
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