Advantages of wide field fluorescence microscopy
1, high resolution
16bit. Because of the confocal imaging of laser and fluorescence and the application of Pinhole to prevent interference from diffracted and scattered light outside the focus, the resolution of confocal microscopes is far beyond the reach of ordinary fluorescence microscopy, which is also popular internationally. One of the reasons.
2, tomography
Confocal takes the electric motor to automatically control the step size so that the minimum moving step on the Z axis can be accurate to 40 nm. This makes the operation of XZt, YZt imaged on the Z axis simple and easy. And the advantages of tomography understand that people will find the advantages when they think about it!
3, a total of 扼 imaging
The function of multi-channel acquisition of images is convenient for confocal to have the function of simultaneously acquiring fluorescent signals of different spectral segments. The layered effect image is obtained by software processing. And through the compensation settings, the effect of Cross talk between different fluorescent signals can be eliminated.
4, differential interference imaging
The difference between the stereoscopic and weak signals of the sample source spectrum is multiplied, so that the image clearly shows different differential expressions.
5, 3D reconstruction
Because of the high resolution and high definition of confocal, it provides a rare advantage for 3D reconstruction. The reconstructed animation comes from the original physiology of the original, showing the most dynamic phenomenon of life mystery, rather than the medical animation in the pure appreciation of the computer! This is not a general microscope can replace.
6. Semi-quantitative and absolute quantification of fluorescence
Fluorescence is strong and weak. It is undoubtedly very comforting for the researchers to provide an exact value to quantify the intensity of fluorescence expression. Isn't it? Especially the absolute quantification of the internal reference method developed in the past two years makes the international The research community once again set off a co-focus boom.
7. Dynamic research
Advantages of ordinary fluorescence microscopy:
1, the operation is relatively simple.
2. The cost of use is relatively low.
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