In the end of September and early October, dry earth seedlings were used for transplanting mustard seedlings. They were transplanted in mid-November and harvested into rice fields after late rice. They were harvested one at a time in early March and early April in the following year, and then planted early rice. Early rice fields after picking mustard are basically used without fertiliser due to the accumulation of fertilizers and rotten vegetables.
Variety selection
Should choose a variety of adaptability, good fertility, strong resistance, late pumping, hollow rate, good yield. Mustard varieties are particularly regional. In the introduction of new varieties, we must conduct large-scale planting on the basis of small-scale planting trials.
Sowing seedlings
Sowing usually takes place from late September to early October. However, early sowing should not be earlier than September 22. For any reason, postponing sowing should not be later than October 8. Seeding is generally done on a sunny or cloudy afternoon. After sowing, 0.5 kg of boron fertilizer is applied per acre seedbed. The boron fertilizer is dissolved by boiled water before application, and evenly mixed with fine soil and the seeds are covered with approximately 0.5 cm thick as cover seed fertilizer. Generally, 50-75 grams of seeds are used per acre of field seed, and 0.75 kg of seeds are sown per acre seedbed (8-10 mu can be transplanted in Daejeon). In the event of drought, in the early morning with clean water or dredged water, the frequency and interval will depend on the drought conditions, so that the surface does not appear dry. Seedlings and time seedlings 2-3 times.
Timely colonization
Seeds planted before September 25th, seedling age about 30-35 days can be transplanted; seedlings after October 3rd, seedling age about 38-43 days; seeding between the two, seedling age about 33-40 Days can be transplanted. When transplanting with soil, cut too long roots with scissors. After planting drought, it is necessary to pay attention to pouring (irrigation) water to drought and ensure that the live trees are seedlings. Normally Daejeon applies 2,000-2,500 kilograms of organic manure per acre, ploughs into the soil, combines 50 kg of compound fertilizer in Mushi, and then prepares the soil. Can be planted 20,000 per acre, spacing 24 cm, spacing 12 cm. Root water was poured immediately after transplanting. It is covered with mulching film to increase the ground temperature and accelerate plant growth.
Field management
Stem mustard should be top dressing according to different cultivation conditions and growth characteristics of stems and leaves. Generally 3 times of top dressing: After the first planting and after the formation of the first leaf ring (before December 15th), generally, the concentration of manure is 1500-1750 kg or urea is 4-5 kg ​​per mu and 1000 kg of water is added. The second top-dressing begins after the stem begins to swell and gradually forms the second and the third leaf ring (mid-January to early February). Generally, 25 kg of ammonium bicarbonate per mu, 20 kg of superphosphate, 5 kg of potassium chloride, and 1500 of water are added. Kilograms poured. The third time before the rapid growth of the stem (in late February), use 25 kg of urea plus 12.5 kg of potassium chloride per acre and pour water. Do not apply or apply nitrogen fertilizer. After 7 days, follow up with the growth. fat. One month before picking, stop top dressing to prevent growth from being too fast and hollow.
If there is a long-term drought before the winter, the water can be ditched once according to the situation, and drained in a timely manner. If there is too much rain in winter, drainage should be drained in time to prevent the flood. In February-March, if there is much rain, the humidity in the field is high, and high temperature and high humidity are easy to cause diseases, drainage and disease prevention work should be done well.
After field transplanting, Datian used to treat the hernia twice. The agent used was 1500 times of enefluramine aqueous solution, 3,000 times of 20% acetamiprid EC or 70% of imidacloprid water dispersible granules. Add thiophanate-methyl or mancozeb and other diseases.
PCR ( Polymerase Chain Reaction)
It provides a way to make more copies of a portion of DNA.
We need to have PCR ingredients that include DNA portion, Buffer solution, Primer+DNA (tap polymerase), and Nucleotides during the preparation step.
In the step of PCR sequence, there are three steps in this section.
Step 1 Denaturation
The addition of heat is needed to separate the two strands of the DNA molecule.
Step 2 Annealing
Temperature, is going to be cool in this step, should allow the primers to bind to the specific segments.
Step 3 DNA Synthesis
To make more copies of the DNA.
Those three steps above keep repeating to make enough copies of the DNA fragments. It is the principle of how PCR machine works.
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