1. Cultivation environment conditions
The growth temperature of Agrocybe aegerita mycelium is 3-35°C, and the optimum growth temperature is 23-28°C. At temperatures above 30°C, mycelial growth weakened or even stopped. The developmental temperature of the fruiting body is 23-26°C, and the optimum temperature is 18-24°C. Higher or lower temperatures will delay the primordial differentiation. According to experiments, when the temperature is about 20°C, the fruiting is best, from budding to maturity about 10 days. The agrocybe aegerita is in the growth stage of mycelia, and the suitable moisture content of the culture material is 60%-65%. The air is relatively when the mycelium grows. Humidity is 65%-70%. Mycelium growth stage does not require light, excessively bright scattered light inhibit mycelial growth. Agrocybe aegypti is aerobic and is very sensitive to CO2. Poor ventilation, high CO2 content, slender stems, blocked cap development, easy to open umbrellas or teratogenicity. The mycelium can grow in the pH range of 4-7, grow best at pH 4.5-5.5, and the mycelium will grow poorly when the pH is lower than 4 or higher than 7.
2, preparation before planting
Agrocybe aegerita has an indoor shelf erecting bag, mushrooming, bagging, soil and mushroom cultivation, and wall cultivation. Spring planting is generally carried out in late February to early March. Inoculation takes place in April and May, and it is cultivated in April and May. In autumn, it is planted at the end of August and early September, and in October-November, it is cultivated. Chai Mushroom bags are usually grown in the shed. Choose a simple mushroom house with high ground, sunny ventilation, convenient transportation, near water source, and no pollution around. The structure is best for brick and tile structure. A bed frame can be set in a mushroom house or a mushroom house. Fengxian's general culture formula is 40% of wood chips, 30% of cotton seed hull, 16% of wheat bran, 8% of corn flour, 4% of rapeseed cake powder, 1% of gypsum, 0.5% of sugar, 0.5% of potassium dihydrogen phosphate, and sulfuric acid. Magnesium 0.1%.
3, bag production
The ingredients were weighed in a conventional manner, the water content was adjusted to about 60%, the feed water ratio was about 1.0:1.2, and the pH before sterilization was about 7. After the water has penetrated evenly and in the fashion bag. Inflated bag cultivation usually uses a low-pressure polyethylene film bag with a specification of 15.00cm55.00cm 0.04cm. Each bag contains 700g of dry material. The specific operation for high-temperature sterilization is to open the valve and heat until the pressure rises to 0.8-1.1. Kg/cm2, discharge gas reaches 107°C, control 2-3h, ensure that the heat inside the pot has enough time to penetrate the bag, the temperature inside and outside the bag is uniform and then warm, so that the pressure rises to 1.5-1.7kg/cm2 , keep 105-120min. start to rise, the fire raging, requiring the center temperature after the ignition within 2h bag 97-100 °C, and then maintain 15-18h [1-2]. Halfway can not stop the fire, can not cool, keep the slow fire, Keep 100°C. The cultivated original species and cultivars were thick and thick, and were inoculated at both ends of the bag when the material was cooled to 28°C. It is also possible to perform punching and inoculation after sterilization. After inoculation, inoculate the wells with clear tape. Each bottle of bacteria can inoculate 25 bags.
4. Fungal management
In the first 3 days after inoculation, room temperature was adjusted to 25-27°C to make mycelia grow robustly. When the mycelium grows to 1/2 the depth of the material, its growth begins to slow down. At this time, loosen the bag mouth to tie the rope, or puncture 1-2 lines of microporous oxygen in the 1.0-1.5cm in front of the hypha growth. The relative humidity of the indoor air during the germination phase should be controlled at about 70%. There must not be excessive light, and doors and windows must be shaded.
5, Lei Lei management
Outdoor mushroom shed method is as follows: the site is organized into a 30cm wide, 15cm high boring machine, bed on a layer of sand, and then spread two layers of film, the bag ends of the bag cut open stacked on a trampoline. The direction in which the bags should be stacked should be consistent with the direction of the doors and windows of the shed. Cut bag and row bag at the same time. Prior to the opening of the fungus bag, 34% carbolic acid solution or deltamethrin (killed) 3000 times solution and dimethoate 2000 times were used to disinfect the bag and disinfested the site. Use a sharp knife to cut the bag's membrane along the tie-knot [3]. After the bag is cut, a brown layer of bacilli will form on the surface around the bag opening as the browning time of the mycelium increases and the color deepens. The normal bacteriostasis was tan and rust brown, shiny, and normal fruiting. Combining with the turning of the bag, the temperature difference is continuously increased for 3-7 days, so that the temperature difference between day and night reaches 8-10°C, until many white granules appear and differentiate into mushroom buds.
6, mushroom management
During the mushrooming period, the temperature of the mushroom shed is maintained at 15-26°C, preferably 20-24°C. After the formation of the primordium, the number of water sprays should be increased to keep the relative humidity of the air between 85% and 90%. In the early stage, water is mainly sprayed into the space and the ground, and water can be directly sprayed into the fruit body in the middle and later stages. During the growth of the fruiting body, the respiration is strong. During the mushrooming period, the mushroom shed must be ventilated to keep the air fresh [4]. The light intensity should be maintained at 500-1000 lx during the mushrooming period. Do not move the mushroom bag at will during the growth, nor can it allow the mushrooms to grow. The light source of the shed is disordered. Agrocybe can be used to collect 3-4 mushroom. After each harvest of a tidal mushroom, the bag is covered with a plastic film, the water is stopped for 7-10 days, and the relative humidity of the air is maintained at 70%-75%. After the wound surface is collected at the mushroom, the budding is performed again according to the above method.
Garden Series
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