Product Name : VIVAlinkTMubiquitin kit ,VIVAlinkTM Protein Ubiquitination Kit
Goods number : VB2952
Verifiable sample types : cell lysates, purified proteins, tissue extracts
Knowledge background :
Ubiquitin (Ub) is a highly conserved protein widely present in eukaryotic cells. It cooperates with ubiquitin-activating enzyme (E1), ubiquitin-binding enzyme (E2), and ubiquitin ligase (E3) to achieve ubiquitination of specific proteins, ultimately achieving the elimination of aging, damage, and misfolded proteins. The normal physiological function of cells plays a very important role. In addition, certain ubiquitination modifications can also achieve functional regulation independent of protein degradation.
Kit introduction :
VIVAlinkTM ubiquitination modification kit is simple and fast, and can efficiently realize ubiquitination modification of cell lysate, tissue lysate and purified protein in vitro environment. Finally, it can be detected by WB, mass spectrometry and biochemical analysis. The effect of the modification; the kit provides a series of E2 binding enzymes, each of which can be used for 4 reactions, so the reagents of the kit can be used for 50 reactions.
Features:
Self-ubiquitination and substrate ubiquitination detection by different E2 binding enzymes;
Develop ubiquitination analysis for specific ubiquitination-mediated processes;
Multiple assays can be performed with each E2 binding enzyme;
The final test can be identified by WB, mass spectrometry, biochemical analysis;
Use range :
Realize the detection of E3 from ubiquitination and substrate ubiquitination;
Appropriate E2 enzymes are judged by a specific E3 or E3-substrate system;
Assessing the activity of the novel enzyme E3 ligase;
Assessing the effect of ubiquitination modification on substrate proteins;
References :
Fulda, S., Rajalingam, K. & Dikic, I. Ubiquitylation in immune disorders and cancer: from molecular mechanisms to therapeutic implications. EMBO molecular medicine 4, 545–56 (2012). PMID: 22730341
Shaid, S., Brandts, CH, Serve, H. & Dikic, I. Ubiquitination and selective autophagy. Cell death and differentiation 20, 21–30 (2013). PMID: 22722335
Husnjak, K. & Dikic, I. Ubiquitin-binding proteins: decoders of ubiquitin-mediated cellular functions. Annual review of biochemistry 81, 291–322 (2012). PMID: 22482907
Komander, D. & Rape, M. The Ubiquitin Code. Annual Review of Biochemistry 81, 203–229 (2012). PMID: 22524316
Spasser, L. & Brik, A. Chemistry and biology of the ubiquitin signal. Angewandte Chemie (International ed. in English) 51, 6840–62 (2012). PMID: 22696461
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